Peddie 2016 Summer Blog Link

http://peddieschoolexp2016.blogspot.com/2016/05/

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Emily Guo, Week 7, Hanging Up the Lab Coat

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Some good news: the plasmids Anna and I made a few weeks ago were sent off for sequencing, and they came back with the correct sequence! The plasmids can now be used in the next round of electroporation.  We are currently still troubleshooting the bisulfite conversion process. I ran a PCR for the DNA samples (non-converted) that we were using on Monday with two sets of primers that we knew would work, and we did indeed have bands of DNA show up on the gel afterwards. Therefore, the quality of the DNA that we were converting wasn’t the problem. I found a bisulfite conversion guide by Zymo Research – which deals a lot in epigenetic research in addition to providing biology research tools for DNA and RNA research and analysis – that provided some tips that we could try out. One was a way to analyze the DNA after bisulfite conversion, which we didn’t do before, by running the gel with the bisulfite-converted product. However, because the DNA becomes so damaged through the conversi...
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