Peddie 2016 Summer Blog Link

http://peddieschoolexp2016.blogspot.com/2016/05/

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Stephanie Wu | Week 3 | More Slicing and Mounting

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06.19.17 - 06.25.17 For the majority of this week I spent my time slicing and mounting the brains Felicia had previously removed from some mice via perfusion after inserting the injections she was working with. The main type of mouse I have worked with are the rAAV2 Delta-Cre mice (which are the controls) and the rAAV2 GFP-Cre mice (which are the experimentals), which are then divided into hetero and homo. From there, Felicia targeted specific regions of the brain, mostly injecting into areas such as the nucleus accumbens (NAc) and the dorsal striatum (DS). She injected both a control virus as well as multiple experimental viruses. By slicing the brains we would eventually be able to image them on the microscope and see the effects of the injection on the synapses of the brains and the different afferent sites as well. (some brain slices (N1C. 87)) (more slices) (some mounted slides of N1C. 125 & 126 brain slices) (what my slide box currently looks like) Bes...
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Emily Guo, Week 7, Hanging Up the Lab Coat

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Some good news: the plasmids Anna and I made a few weeks ago were sent off for sequencing, and they came back with the correct sequence! The plasmids can now be used in the next round of electroporation.  We are currently still troubleshooting the bisulfite conversion process. I ran a PCR for the DNA samples (non-converted) that we were using on Monday with two sets of primers that we knew would work, and we did indeed have bands of DNA show up on the gel afterwards. Therefore, the quality of the DNA that we were converting wasn’t the problem. I found a bisulfite conversion guide by Zymo Research – which deals a lot in epigenetic research in addition to providing biology research tools for DNA and RNA research and analysis – that provided some tips that we could try out. One was a way to analyze the DNA after bisulfite conversion, which we didn’t do before, by running the gel with the bisulfite-converted product. However, because the DNA becomes so damaged through the conversi...
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Mia Salas, Week 5+1 day of 6, Real-Time in Lab

This past week I gave a lab presentation at our meeting.   Kate and I both presented separately, and we each had to make a power point about our research.   I picked up donuts from Wawa on the walk over and passed them around before my presentation.   It went great, and my PI was very happy with the work I did over the summer.   I presented on NPC, starting with a basic outline of the disease and the goal of research moving forward, and then I displayed multiple excel graphs comparing the results when treatment began when the cats were 8 weeks old, 12 weeks old, and 16 weeks old.   I compared weight gain, a liver enzyme called ALT, and bilirubin, which is released when red blood cells are broken down, and then is carried to the liver, made into bile, and excreted out of the body.   A main conclusion that I and the people from my lab derived from my research was that there is not a substantial difference between cats who started treatment at 8 weeks old...
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