Peddie 2016 Summer Blog Link

http://peddieschoolexp2016.blogspot.com/2016/05/

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Emily Guo, Week 7, Hanging Up the Lab Coat

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Some good news: the plasmids Anna and I made a few weeks ago were sent off for sequencing, and they came back with the correct sequence! The plasmids can now be used in the next round of electroporation.  We are currently still troubleshooting the bisulfite conversion process. I ran a PCR for the DNA samples (non-converted) that we were using on Monday with two sets of primers that we knew would work, and we did indeed have bands of DNA show up on the gel afterwards. Therefore, the quality of the DNA that we were converting wasn’t the problem. I found a bisulfite conversion guide by Zymo Research – which deals a lot in epigenetic research in addition to providing biology research tools for DNA and RNA research and analysis – that provided some tips that we could try out. One was a way to analyze the DNA after bisulfite conversion, which we didn’t do before, by running the gel with the bisulfite-converted product. However, because the DNA becomes so damaged through the conversi...
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Manas Kaushik Week 6

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One of Ben's Plates I realized for some reason my final blog did not save when I tried uploading it before so here it is now. This was my final week in lab and it came by so fast. I was not ready to leave at all as it had become part of my daily life. The schedule had become so routine and I enjoyed working in a university lab. Ben had been away for two weeks at a conference in England so he had 6 plates of DNA that he needed for genotyping. I planned on finishing most of them this week so that he would not have too much to finish up the rest of the summer. He was trying to work on two projects simultaneously so by completing some of the plates I would make his life a little bit easier.  Loading dye for gel electrophoresis  I ran two plates each day.For each plate I did a pcr which took roughly 2 hours. While the pcr was finishing up, I would make a 1-1.5% gel to run the DNA. After adding the ethidium bromide to the gel, I would let it mix for 10 minutes ...
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