Peddie 2016 Summer Blog Link

http://peddieschoolexp2016.blogspot.com/2016/05/

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Diana King, Week 2, Experiments and Exploring

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I have been continuing to measure the pup call but this week I have finally started to find some consistencies between ages. I am optimistic that this means something and will hold true throughout the other samples. Nevertheless, I graphed the data and recorded the average pup call of p3, p5 and p8 ages. This will help determine how the mother is recognizing the USVs (ultrasonic vocalizations). Previously I had been doing mostly the pup call recordings and watching the brain related aspect of my grad student's project, but this week I transitioned into the behavioral.  When learning anything, the brain takes a singular experience and is able to generalize this to accommodate later experiences like this. When a maternal mouse hears their pup cry they learn the sound of the cry and are able to recognize that cry no matter which pup it is coming from.  For example, when an English speaker hears the word "hello" they are able to understand the meaning no ma...
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Mia Salas, Week 5+1 day of 6, Real-Time in Lab

This past week I gave a lab presentation at our meeting.   Kate and I both presented separately, and we each had to make a power point about our research.   I picked up donuts from Wawa on the walk over and passed them around before my presentation.   It went great, and my PI was very happy with the work I did over the summer.   I presented on NPC, starting with a basic outline of the disease and the goal of research moving forward, and then I displayed multiple excel graphs comparing the results when treatment began when the cats were 8 weeks old, 12 weeks old, and 16 weeks old.   I compared weight gain, a liver enzyme called ALT, and bilirubin, which is released when red blood cells are broken down, and then is carried to the liver, made into bile, and excreted out of the body.   A main conclusion that I and the people from my lab derived from my research was that there is not a substantial difference between cats who started treatment at 8 weeks old...
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Jerry Wang, Week 3, PCR, PCR, PCR and more PCR

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Week3:                 This week was really exciting since my primer finally showed up at the doorstep right when I entered the lab on Monday. The first step of processing such primer is to reconstitute it. I was really confused by the word “reconstitution” since I have never heard of such word in my entire life. This is actually a common experience since research labs often use very advanced word for very simple idea. And in this case, reconstituting a primer literally means adding water to dissolve the DNA piece that was laying at the bottom of the test tube. I “reconstitute” the primer in the fancy fume hood we got in tissue culture lab. A fume hood is basically a sterile environment that would prevent particles from entering or exiting the hood. Here, since the primer is very delicate and we would not want any contamination to it, we performed the reconstitution process with molecular biology grade water (...
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