Peddie 2016 Summer Blog Link

http://peddieschoolexp2016.blogspot.com/2016/05/

Comments

Post a Comment

Popular posts from this blog

Emily Guo, Week 7, Hanging Up the Lab Coat

Image
Some good news: the plasmids Anna and I made a few weeks ago were sent off for sequencing, and they came back with the correct sequence! The plasmids can now be used in the next round of electroporation.  We are currently still troubleshooting the bisulfite conversion process. I ran a PCR for the DNA samples (non-converted) that we were using on Monday with two sets of primers that we knew would work, and we did indeed have bands of DNA show up on the gel afterwards. Therefore, the quality of the DNA that we were converting wasn’t the problem. I found a bisulfite conversion guide by Zymo Research – which deals a lot in epigenetic research in addition to providing biology research tools for DNA and RNA research and analysis – that provided some tips that we could try out. One was a way to analyze the DNA after bisulfite conversion, which we didn’t do before, by running the gel with the bisulfite-converted product. However, because the DNA becomes so damaged through the conversi...
Read more

Jerry Wang, Week 1, Finally starting my summer at UPenn

Week1:                 After a few weeks, I have finally arrived Philadelphia. Things went a bit side track since my kitty scratched me on accident back at home and I need to get vaccinated which would not allow me to go to US till July 18 th . One thing led to another and the result is that I arrived at my lab two weeks later than the plan. But anyways, after the long wait I was really excited to meet my lab member and P.I. My P.I. is currently on a personal vocation in Europe right now and will be back next Monday. Therefore, this week, I worked alongside of Dr. Gunan, the staff member of my lab. First day was always the toughest one. But with the help of Apple map and Upenn’s website, I successfully located Dr. Mason’s lab. The first “task” I had for the lab was making a 0.5 percent agarose gel. It may doesn’t sound hard to all of you who just finished biology but my last biology-related experience was almo...
Read more

Manas Kaushik Week 6

Image
One of Ben's Plates I realized for some reason my final blog did not save when I tried uploading it before so here it is now. This was my final week in lab and it came by so fast. I was not ready to leave at all as it had become part of my daily life. The schedule had become so routine and I enjoyed working in a university lab. Ben had been away for two weeks at a conference in England so he had 6 plates of DNA that he needed for genotyping. I planned on finishing most of them this week so that he would not have too much to finish up the rest of the summer. He was trying to work on two projects simultaneously so by completing some of the plates I would make his life a little bit easier.  Loading dye for gel electrophoresis  I ran two plates each day.For each plate I did a pcr which took roughly 2 hours. While the pcr was finishing up, I would make a 1-1.5% gel to run the DNA. After adding the ethidium bromide to the gel, I would let it mix for 10 minutes ...
Read more