Kabir Grewal, UPitt Lab, End of Week 4
I ended my last blog post with writing about when I injected TMSC’s and CSSC’s into well plates with the extracellular matrix of bovine corneal endothelial cells (BCE cells) , which brings me to the work I completed last Thursday and Friday. Last Thursday was a busy day for me. After arriving at the lab at the usual time of 9:30, I checked the growth of the newly injected stem cells mixed with the extracellular matrix of lysed BCE cells. As expected, the stem cells had not attached to the bottom of the well plates, and were free floating in the DF10 growth medium. Today, my task was to make a new growth medium for these cells, as well as create the variables for the experiment. The new growth medium I made comprised of a glucose-rich base, fetal bovine serum (FBS), as well as ROCK inhibitor (short for Rho Kinase inhibitor). The purpose of this inhibitor is to prevent excessive apoptosis among the cells, and promote the cells to grow and retain their struc...