Stephanie Wu | Week 3 | More Slicing and Mounting
06.19.17 - 06.25.17 For the majority of this week I spent my time slicing and mounting the brains Felicia had previously removed from some mice via perfusion after inserting the injections she was working with. The main type of mouse I have worked with are the rAAV2 Delta-Cre mice (which are the controls) and the rAAV2 GFP-Cre mice (which are the experimentals), which are then divided into hetero and homo. From there, Felicia targeted specific regions of the brain, mostly injecting into areas such as the nucleus accumbens (NAc) and the dorsal striatum (DS). She injected both a control virus as well as multiple experimental viruses. By slicing the brains we would eventually be able to image them on the microscope and see the effects of the injection on the synapses of the brains and the different afferent sites as well. (some brain slices (N1C. 87)) (more slices) (some mounted slides of N1C. 125 & 126 brain slices) (what my slide box currently looks like) Bes...
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